PrimePCR™ SYBR® Green Assay: AC093510.2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040286

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AC093510.2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC093510.2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037563
Assay Design:   Intron-spanning
Chromosome Location:   5:89757310-89769738question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000552923 ENST00000317506 ENST00000448631 ENST00000602677 ENST00000411657 ENST00000536251 ENST00000406771 ENST00000430276 ENST00000373950 ENST00000452261 ENST00000476080 ENST00000292431 ENST00000585663 ENST00000225426 ENST00000579088 ENST00000519357 ENST00000370994 ENST00000218758 ENST00000412435 ENST00000592828 ENST00000433365 ENST00000397293 ENST00000252487 ENST00000426677 ENST00000405636 ENST00000445941 ENST00000227880 ENST00000378814 ENST00000502297 ENST00000259711 ENST00000378843 ENST

Gene Information

Description Not Available

Gene Symbol:   AC093510.2
Gene Name:   metallo-beta-lactamase domain-containing protein 2
Aliases:   DKFZp686P15118, MGC46734
RefSeq:   NC_000005.9 NT_006713.15
Ensembl:   ENSG00000259131
Entrez:   153364
Chromosome Mapping:   5q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.290000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download