PrimePCR™ SYBR® Green Assay: AF196779.12, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040255

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AF196779.12, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AF196779.12, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037532
Assay Design:   Intron-spanning
Chromosome Location:   X:48935741-48937484question
Amplicon Length:   76
Splice Variants Targeted:   ENST00000376372 ENST00000322995 ENST00000485908 ENST00000473974 ENST00000376368 ENST00000396681 ENST00000471338 ENST00000475880 ENST00000476728 ENST00000553851 ENST00000376358 ENST00000392870 ENST00000600785 ENST00000602067 ENST00000596615 ENST00000601290 ENST00000600730 ENST00000601603 ENST00000602268 ENST00000601175 ENST00000599262 ENST00000601827 ENST00000599940

Gene Information

Description Not Available

Gene Symbol:   AF196779.12
Gene Name:   Uncharacterized protein; Uncharacterized protein
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000250232
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.930000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download