PrimePCR™ SYBR® Green Assay: LRRC37A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040210

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LRRC37A2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LRRC37A2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037487
Assay Design:   Intron-spanning
Chromosome Location:   17:44592625-44597622question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000547334 ENST00000547001 ENST00000549105 ENST00000597888 ENST00000600392 ENST00000552021 ENST00000543378 ENST00000327564 ENST00000584368 ENST00000341671 ENST00000394713 ENST00000495880 ENST00000296483 ENST00000584306 ENST00000584788 ENST00000319651 ENST00000393465 ENST00000320254 ENST00000576629 ENST00000333412 ENST00000591674 ENST00000341156 ENST00000262809 ENST00000340648 ENST00000341552 ENST00000441968 ENST00000453220 ENST00000433935 ENST00000592813 ENST00000573751 ENST00000269289 ENST

Gene Information

Description Not Available

Gene Symbol:   LRRC37A2
Gene Name:   leucine rich repeat containing 37, member A2
Aliases:   FLJ45049
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000238083
Entrez:   474170
Chromosome Mapping:   17q21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996000
y-intercept 35.920000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download