PrimePCR™ SYBR® Green Assay: KIFC1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040208

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIFC1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIFC1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037485
Assay Design:   Intron-spanning
Chromosome Location:   6:33359734-33366105question
Amplicon Length:   190
Splice Variants Targeted:   ENST00000409548 ENST00000317446 ENST00000445819 ENST00000528094 ENST00000313777 ENST00000445575 ENST00000374523 ENST00000411963 ENST00000438447 ENST00000282493 ENST00000448818 ENST00000426791 ENST00000392693 ENST00000531738 ENST00000263577 ENST00000262525 ENST00000166139 ENST00000264560 ENST00000338448 ENST00000373091 ENST00000367090 ENST00000329468 ENST00000399052 ENST00000252997 ENST00000317398 ENST00000596939 ENST00000335820 ENST00000428849 ENST00000450504 ENST00000338950 ENST00000360066 ENST

Gene Information

Description Not Available

Gene Symbol:   KIFC1
Gene Name:   kinesin family member C1
Aliases:   HSET, KNSL2, MGC1202, MGC149736, MGC149737
RefSeq:   NC_000006.11 NT_007592.15 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000237649
Entrez:   3833
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.210000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download