PrimePCR™ SYBR® Green Assay: ATF6B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040117

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATF6B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATF6B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037394
Assay Design:   Intron-spanning
Chromosome Location:   6:32087709-32088646question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000298854 ENST00000453812 ENST00000394468 ENST00000341723 ENST00000440868 ENST00000435768 ENST00000425571 ENST00000347300 ENST00000296266 ENST00000507564 ENST00000504021 ENST00000349441 ENST00000348417 ENST00000431818 ENST00000440957 ENST00000373302 ENST00000373299 ENST00000293709 ENST00000383156 ENST00000380599 ENST00000311403 ENST00000427136 ENST00000439240 ENST00000377765 ENST00000539465 ENST00000256593 ENST00000334456 ENST00000376450 ENST00000544570 ENST00000418754 ENST00000540345 ENST

Gene Information

The protein encoded by this gene is a transcription factor in the unfolded protein response (UPR) pathway during ER stress. Either as a homodimer or as a heterodimer with ATF6-alpha the encoded protein binds to the ER stress response element interacting with nuclear transcription factor Y to activate UPR target genes. The protein is normally found in the membrane of the endoplasmic reticulum; however under ER stress the N-terminal cytoplasmic domain is cleaved from the rest of the protein and translocates to the nucleus. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2008]

Gene Symbol:   ATF6B
Gene Name:   activating transcription factor 6 beta
Aliases:   CREB-RP, CREBL1, FLJ10066, G13
RefSeq:   NC_000006.11 NG_000013.3 NG_004658.2 NG_005163.2 NT_007592.15 NT_113891.2 NT_167245.1 NT_167247.1
Ensembl:   ENSG00000213676
Entrez:   1388
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download