PrimePCR™ SYBR® Green Assay: MICB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0040061

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MICB, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MICB, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037338
Assay Design:   Intron-spanning
Chromosome Location:   6:31475268-31477664question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000374690 ENST00000442104 ENST00000451603 ENST00000438928 ENST00000415525 ENST00000414473 ENST00000423443 ENST00000546529 ENST00000451789 ENST00000443156 ENST00000420259 ENST00000418465 ENST00000432479 ENST00000417943 ENST00000552236 ENST00000428059 ENST00000456351 ENST00000449934 ENST00000421350 ENST00000399150 ENST00000252229 ENST00000538442 ENST00000307522 ENST00000436531 ENST00000436655 ENST00000437651 ENST00000383514 ENST00000417899 ENST00000427477 ENST00000446505 ENST00000420744 ENST

Gene Information

This gene encodes a heavily glycosylated protein which is a ligand for the NKG2D type II receptor. Binding of the ligand activates the cytolytic response of natural killer (NK) cells CD8 alphabeta T cells and gammadelta T cells which express the receptor. This protein is stress-induced and is similar to MHC class I molecules; however it does not associate with beta-2-microglobulin or bind peptides. [provided by RefSeq Jul 2008]

Gene Symbol:   MICB
Gene Name:   MHC class I polypeptide-related sequence B
Aliases:   PERB11.2
RefSeq:   NC_000006.11 NG_021405.1 NT_007592.15 NT_113891.2 NT_167244.1 NT_167245.1 NT_167246.1 NT_167247.1 NT_167248.1 NT_167249.1
Ensembl:   ENSG00000204516
Entrez:   4277
Chromosome Mapping:   6p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.920000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download