PrimePCR™ SYBR® Green Assay: NBPF4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039973

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: NBPF4, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NBPF4, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037250
Assay Design:   Intron-spanning
Chromosome Location:   1:108771697-108774939question
Amplicon Length:   114
Splice Variants Targeted:   ENST00000237536 ENST00000456801 ENST00000221086 ENST00000374087 ENST00000344348 ENST00000374082 ENST00000438493 ENST00000452682 ENST00000443446 ENST00000430396 ENST00000414907 ENST00000370038 ENST00000415641 ENST00000495380 ENST00000531446 ENST00000444143 ENST00000370040 ENST00000294652 ENST00000577819 ENST00000581486 ENST00000585132 ENST00000579039 ENST00000578454 ENST00000583565 ENST00000585056 ENST00000580070

Gene Information

Description Not Available

Gene Symbol:   NBPF4
Gene Name:   neuroblastoma breakpoint family, member 4
Aliases:   FLJ32833
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000196427
Entrez:   148545
Chromosome Mapping:   1p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.490000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download