PrimePCR™ SYBR® Green Assay: AC022415.1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039961

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: AC022415.1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: AC022415.1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037238
Assay Design:   Intron-spanning
Chromosome Location:   19:12221135-12222093question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000454339 ENST00000357901 ENST00000323169 ENST00000419886 ENST00000344980 ENST00000550507 ENST00000455504 ENST00000552904 ENST00000478765 ENST00000547560 ENST00000550745 ENST00000346932 ENST00000350527 ENST00000308713 ENST00000547628 ENST00000602107 ENST00000430298 ENST00000596883 ENST00000339302 ENST00000334213 ENST00000418866 ENST00000455799 ENST00000439556 ENST00000542938 ENST00000355738 ENST00000293725 ENST00000595977 ENST00000601858 ENST00000397732 ENST00000455490 ENST00000428311 ENST

Gene Information

Description Not Available

Gene Symbol:   AC022415.1
Gene Name:   Zinc finger protein 788
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000188474
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.020000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download