PrimePCR™ SYBR® Green Assay: TBC1D3F, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039931

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TBC1D3F, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TBC1D3F, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037208
Assay Design:   Intron-spanning
Chromosome Location:   17:36288661-36290605question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000373063 ENST00000409208 ENST00000373057 ENST00000307972 ENST00000373060 ENST00000316099 ENST00000415691 ENST00000258341 ENST00000454519 ENST00000398801 ENST00000336331 ENST00000457979 ENST00000308078 ENST00000451448 ENST00000394359 ENST00000455054 ENST00000535446 ENST00000400684 ENST00000394453 ENST00000592614 ENST00000341264 ENST00000330458 ENST00000535592 ENST00000535805 ENST00000257951 ENST00000521271 ENST00000198765 ENST00000311127 ENST00000327454 ENST00000505415 ENST00000378174 ENST

Gene Information

Description Not Available

Gene Symbol:   TBC1D3F
Gene Name:   TBC1 domain family, member 3F
Aliases:   FLJ56187, FLJ58835, TBC1D3
RefSeq:   NC_000017.10 NT_010783.15 NW_003315949.1
Ensembl:   ENSG00000185128
Entrez:   84218
Chromosome Mapping:   17q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.250000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download