PrimePCR™ SYBR® Green Assay: SPACA5B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039869

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: SPACA5B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: SPACA5B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037146
Assay Design:   Intron-spanning
Chromosome Location:   X:47990728-47991857question
Amplicon Length:   166
Splice Variants Targeted:   ENST00000359314 ENST00000389054 ENST00000520569 ENST00000448451 ENST00000398562 ENST00000389057 ENST00000398566 ENST00000398557 ENST00000253811 ENST00000308775 ENST00000515359 ENST00000545947 ENST00000541308 ENST00000539901 ENST00000538711 ENST00000266991 ENST00000376940 ENST00000304355 ENST00000304270 ENST00000333141 ENST00000256190 ENST00000262715 ENST00000556935 ENST00000545983 ENST00000421182 ENST00000323926 ENST00000336916 ENST00000357867 ENST00000354785 ENST00000446046 ENST00000443816 ENST

Gene Information

Description Not Available

Gene Symbol:   SPACA5B
Gene Name:   sperm acrosome associated 5B
Aliases:   SPACA5
RefSeq:   NC_000023.10 NT_079573.4
Ensembl:   ENSG00000171478
Entrez:   729201
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download