PrimePCR™ SYBR® Green Assay: NBPF11, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039828

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NBPF11, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NBPF11, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037105
Assay Design:   Intron-spanning
Chromosome Location:   1:146041006-146046359question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000412624 ENST00000369365 ENST00000369190 ENST00000369372 ENST00000375552 ENST00000338347 ENST00000440491 ENST00000281815 ENST00000369339 ENST00000448873 ENST00000369338 ENST00000342960 ENST00000491285 ENST00000423459 ENST00000479926 ENST00000339388 ENST00000369323 ENST00000401009 ENST00000442909 ENST00000446760 ENST00000309471 ENST00000439206 ENST00000369226 ENST00000369228 ENST00000310701 ENST00000444640 ENST00000431121 ENST00000436356 ENST00000448574 ENST00000458135 ENST00000392972 ENST

Gene Information

Description Not Available

Gene Symbol:   NBPF11
Gene Name:   neuroblastoma breakpoint family, member 11
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_167185.1
Ensembl:   ENSG00000152042
Entrez:   200030
Chromosome Mapping:   1q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 35.820000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download