PrimePCR™ SYBR® Green Assay: TMEM236, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039826

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: TMEM236, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: TMEM236, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037103
Assay Design:   Intron-spanning
Chromosome Location:   10:17818056-17838018question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000569785 ENST00000254323 ENST00000590508 ENST00000440752 ENST00000358127 ENST00000325722 ENST00000381280 ENST00000220325 ENST00000343638 ENST00000302904 ENST00000373644 ENST00000337685 ENST00000377495 ENST00000338221 ENST00000480516 ENST00000457860 ENST00000377819 ENST00000546292 ENST00000538115 ENST00000542585 ENST00000546213

Gene Information

Description Not Available

Gene Symbol:   TMEM236
Gene Name:   transmembrane protein 236
Aliases:   Not Available
RefSeq:   Not Available
Ensembl:   ENSG00000148483
Chromosome Mapping:   Not Available

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.994800
y-intercept 36.770000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download