PrimePCR™ SYBR® Green Assay: OPN1MW, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039823

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: OPN1MW, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: OPN1MW, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037100
Assay Design:   Intron-spanning
Chromosome Location:   X:153455682-153457293question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000367505 ENST00000373233 ENST00000369951 ENST00000442922 ENST00000334956 ENST00000550549 ENST00000369935 ENST00000430054 ENST00000309447 ENST00000369929 ENST00000430419 ENST00000259154 ENST00000597195 ENST00000595220 ENST00000595290 ENST00000596998 ENST00000599007 ENST00000594463 ENST00000599405

Gene Information

This gene encodes for a light absorbing visual pigment of the opsin gene family. The encoded protein is called green cone photopigment or medium-wavelength sensitive opsin. Opsins are G-protein coupled receptors with seven transmembrane domains an N-terminal extracellular domain and a C-terminal cytoplasmic domain. The long-wavelength opsin gene and multiple copies of the medium-wavelength opsin gene are tandemly arrayed on the X chromosome and frequent unequal recombination and gene conversion may occur between these sequences. X chromosomes may have fusions of the medium- and long-wavelength opsin genes or may have more than one copy of these genes. Defects in this gene are the cause of deutanopic colorblindness. [provided by RefSeq Mar 2009]

Gene Symbol:   OPN1MW
Gene Name:   opsin 1 (cone pigments), medium-wave-sensitive
Aliases:   CBBM, CBD, COD5, GCP, GOP, MGC176615, MGC177321, MGC198468, MGC198469, OPN1MW1, OPN1MW2
RefSeq:   NC_000023.10 NG_011606.1 NT_167198.1
Ensembl:   ENSG00000147380
Entrez:   2652
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.690000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download