PrimePCR™ SYBR® Green Assay: MAGEA9B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039800

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: MAGEA9B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: MAGEA9B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0037077
Assay Design:   Intron-spanning
Chromosome Location:   X:148665020-148669088question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000262525 ENST00000367069 ENST00000449822 ENST00000252655 ENST00000297262 ENST00000439158 ENST00000396838 ENST00000414429 ENST00000344279 ENST00000453745 ENST00000243314 ENST00000298974 ENST00000522429 ENST00000519822 ENST00000523516 ENST00000366676 ENST00000366675 ENST00000258281 ENST00000595065 ENST00000593349 ENST00000598469 ENST00000594744 ENST00000595910

Gene Information

MAGEA9B is a duplication of the MAGEA9 gene (MIM 300342) on chromosome Xq28. The 2 copies are separated by about 194 kb (Hartz 2009).[supplied by OMIM Mar 2009]

Gene Symbol:   MAGEA9B
Gene Name:   melanoma antigen family A, 9B
Aliases:   MAGEA9
RefSeq:   NC_000023.10 NT_011681.16
Ensembl:   ENSG00000123584
Entrez:   728269
Chromosome Mapping:   Xq28

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 36.260000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download