PrimePCR™ SYBR® Green Assay: PDCD6IP, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039499

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: PDCD6IP, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: PDCD6IP, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036775
Assay Design:   Intron-spanning
Chromosome Location:   3:33863536-33866787question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000307296 ENST00000413073 ENST00000457054

Gene Information

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis in intralumenal endosomal vesicle formation and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition the product of this gene binds to the product of the PDCD6 gene a protein required for apoptosis in a calcium-dependent manner. This gene product also binds to endophilins proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq Jan 2012]

Gene Symbol:   PDCD6IP
Gene Name:   programmed cell death 6 interacting protein
Aliases:   AIP1, Alix, DRIP4, HP95, MGC17003
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000170248
Entrez:   10015
Chromosome Mapping:   3p22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.190000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download