PrimePCR™ SYBR® Green Assay: NLRP3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039418

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: NLRP3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: NLRP3, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036694
Assay Design:   Intron-spanning
Chromosome Location:   1:247582325-247586626question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000391828 ENST00000336119 ENST00000348069 ENST00000366496 ENST00000391827 ENST00000366497

Gene Information

This gene encodes a pyrin-like protein containing a pyrin domain a nucleotide-binding site (NBS) domain and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC which contains a caspase recruitment domain and is a member of the NALP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling and it plays a role in the regulation of inflammation the immune response and apoptosis. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS) Muckle-Wells syndrome (MWS) chronic infantile neurological cutaneous and articular (CINCA) syndrome and neonatal-onset multisystem inflammatory disease (NOMID). Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq Oct 2008]

Gene Symbol:   NLRP3
Gene Name:   NLR family, pyrin domain containing 3
Aliases:   AGTAVPRL, AII, AII/AVP, AVP, C1orf7, CIAS1, CLR1.1, FCAS, FCU, FLJ95925, MWS, NALP3, PYPAF1
RefSeq:   NC_000001.10 NT_167186.1 NG_007509.2
Ensembl:   ENSG00000162711
Entrez:   114548
Chromosome Mapping:   1q44

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.420000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download