PrimePCR™ SYBR® Green Assay: FH, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0039077

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FH, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FH, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0036353
Assay Design:   Intron-spanning
Chromosome Location:   1:241675356-241676964question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000366560

Gene Information

The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle or Krebs cycle and catalyzes the formation of L-malate from fumarate. It exists in both a cytosolic form and an N-terminal extended form differing only in the translation start site used. The N-terminal extended form is targeted to the mitochondrion where the removal of the extension generates the same form as in the cytoplasm. It is similar to some thermostable class II fumarases and functions as a homotetramer. Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. [provided by RefSeq Jul 2008]

Gene Symbol:   FH
Gene Name:   fumarate hydratase
Aliases:   HLRCC, LRCC, MCL, MCUL1
RefSeq:   NC_000001.10 NG_012338.1 NT_167186.1
Ensembl:   ENSG00000091483
Entrez:   2271
Chromosome Mapping:   1q42.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996100
y-intercept 36.710000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download