PrimePCR™ SYBR® Green Assay: ATP7B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ATP7B, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ATP7B, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0023257
Assay Design:   Intron-spanning
Chromosome Location:   13:52542712-52544726question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000242839 ENST00000400366 ENST00000344297 ENST00000448424 ENST00000418097

Gene Information

This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains an ATPase consensus sequence a hinge domain a phosphorylation site and at least 2 putative copper-binding sites. This protein functions as a monomer exporting copper out of the cells such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants encoding different isoforms with distinct cellular localizations have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq Jul 2008]

Gene Symbol:   ATP7B
Gene Name:   ATPase, Cu++ transporting, beta polypeptide
Aliases:   PWD, WC1, WD, WND
RefSeq:   NG_028038.1 NC_000013.10 NG_008806.1 NT_024524.14
Ensembl:   ENSG00000123191
Entrez:   540
Chromosome Mapping:   13q14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.070000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download