This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033676
PrimePCR™ PreAmp for SYBR® Green Assay: CLK1, Human
PrimePCR™ Template for SYBR® Green Assay: CLK1, Human
This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors including serine/arginine rich proteins. Therefore the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jun 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.