This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033647
PrimePCR™ PreAmp for SYBR® Green Assay: TIMP2, Human
PrimePCR™ Template for SYBR® Green Assay: TIMP2, Human
This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.