PrimePCR™ SYBR® Green Assay: CELA2B, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022866
Assay Design:   Intron-spanning
Chromosome Location:   1:15802989-15807647question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000375910 ENST00000422901 ENST00000375909

Gene Information

Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1 2 2A 2B 3A and 3B. Like most of the human elastases elastase 2B is secreted from the pancreas as a zymogen. In other species elastase 2B has been shown to preferentially cleave proteins after leucine methionine and phenylalanine residues. [provided by RefSeq Jul 2008]

Gene Symbol:   CELA2B
Gene Name:   chymotrypsin-like elastase family, member 2B
Aliases:   ELA2B, MGC142100, MGC142102, MGC97052
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000215704
Entrez:   51032
UniGene:   Hs.631871
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.590000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download