PrimePCR™ SYBR® Green Assay: RC3H1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022347
Assay Design:   Intron-spanning
Chromosome Location:   1:173907960-173910440question
Amplicon Length:   58
Splice Variants Targeted:   ENST00000367696 ENST00000367694 ENST00000258349

Gene Information

RC3H1 or roquin encodes a highly conserved member of the RING type ubiquitin ligase protein family (Vinuesa et al. 2005 [PubMed 15917799]). The roquin protein is distinguished by the presence of a CCCH zinc finger found in RNA-binding proteins and localization to cytosolic RNA granules implicated in regulating mRNA translation and stability.[supplied by OMIM Mar 2008]

Gene Symbol:   RC3H1
Gene Name:   ring finger and CCCH-type domains 1
Aliases:   KIAA2025, RNF198, ROQUIN
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000135870
Entrez:   149041
UniGene:   Hs.30258
Chromosome Mapping:   1q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.260000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download