PrimePCR™ SYBR® Green Assay: RNF13, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022116
Assay Design:   Intron-spanning
Chromosome Location:   3:149589876-149613292question
Amplicon Length:   69
Splice Variants Targeted:   ENST00000392894 ENST00000344229 ENST00000491086 ENST00000467977 ENST00000468648 ENST00000459632 ENST00000466795 ENST00000490631 ENST00000361785 ENST00000543506

Gene Information

The protein encoded by this gene contains a RING zinc finger a motif known to be involved in protein-protein interactions. The specific function of this gene has not yet been determined. Alternatively spliced transcript variants that encode the same protein have been reported. A pseudogene which is also located on chromosome 3 has been defined for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   RNF13
Gene Name:   ring finger protein 13
Aliases:   FLJ93817, MGC13689, RZF
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000082996
Entrez:   11342
UniGene:   Hs.12333
Chromosome Mapping:   3q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.910000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download