PrimePCR™ SYBR® Green Assay: FKBP6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033295

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: FKBP6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: FKBP6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0022110
Assay Design:   Intron-spanning
Chromosome Location:   7:72744272-72745702question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000431982 ENST00000413573 ENST00000252037

Gene Information

The protein encoded by this gene is a member of the immunophilin protein family which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein may have cis-trans prolyl isomerase activity and binds to clathrin heavy chain and heat shock protein 72. This gene is found to be deleted in Williams syndrome and the orthologous gene in mouse is essential for fertility and homologous pairing in male meiosis. [provided by RefSeq Sep 2008]

Gene Symbol:   FKBP6
Gene Name:   FK506 binding protein 6, 36kDa
Aliases:   FKBP36, MGC87179, PPIase
RefSeq:   NC_000007.13 NG_023242.1 NT_007933.15
Ensembl:   ENSG00000077800
Entrez:   8468
Chromosome Mapping:   7q11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.800000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download