This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033295
PrimePCR™ PreAmp for SYBR® Green Assay: FKBP6, Human
PrimePCR™ Template for SYBR® Green Assay: FKBP6, Human
The protein encoded by this gene is a member of the immunophilin protein family which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. The protein may have cis-trans prolyl isomerase activity and binds to clathrin heavy chain and heat shock protein 72. This gene is found to be deleted in Williams syndrome and the orthologous gene in mouse is essential for fertility and homologous pairing in male meiosis. [provided by RefSeq Sep 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.