This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033277
PrimePCR™ PreAmp for SYBR® Green Assay: MATR3, Human
PrimePCR™ Template for SYBR® Green Assay: MATR3, Human
This locus encodes a nuclear matrix protein. Mutations at this locus have been associated with distal myopathy 2 which often includes vocal cord and pharyngeal weakness. Alternatively spliced transcript variants including read-through transcripts with an upstream locus have been described. Related pseuodgenes have been defined on chr1 and chrX.[provided by RefSeq Mar 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.