PrimePCR™ SYBR® Green Assay: MTFMT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033249

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021950
Assay Design:   Intron-spanning
Chromosome Location:   15:65295551-65298457question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000220058

Gene Information

The protein encoded by this nuclear gene localizes to the mitochondrion where it catalyzes the formylation of methionyl-tRNA. [provided by RefSeq Jun 2011]

Gene Symbol:   MTFMT
Gene Name:   mitochondrial methionyl-tRNA formyltransferase
Aliases:   FMT1
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000103707
Entrez:   123263
UniGene:   Hs.531615
Chromosome Mapping:   15q22.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.820000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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