PrimePCR™ SYBR® Green Assay: LAMC2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021924
Assay Design:   Intron-spanning
Chromosome Location:   1:183155439-183177067question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000493293 ENST00000264144 ENST00000537180

Gene Information

Laminins a family of extracellular matrix glycoproteins are the major noncollagenous constituent of basement membranes. They have been implicated in a wide variety of biological processes including cell adhesion differentiation migration signaling neurite outgrowth and metastasis. Laminins composed of 3 non identical chains: laminin alpha beta and gamma (formerly A B1 and B2 respectively) have a cruciform structure consisting of 3 short arms each formed by a different chain and a long arm composed of all 3 chains. Each laminin chain is a multidomain protein encoded by a distinct gene. Several isoforms of each chain have been described. Different alpha beta and gamma chain isomers combine to give rise to different heterotrimeric laminin isoforms which are designated by Arabic numerals in the order of their discovery i.e. alpha1beta1gamma1 heterotrimer is laminin 1. The biological functions of the different chains and trimer molecules are largely unknown but some of the chains have been shown to differ with respect to their tissue distribution presumably reflecting diverse functions in vivo. This gene encodes the gamma chain isoform laminin gamma 2. The gamma 2 chain formerly thought to be a truncated version of beta chain (B2t) is highly homologous to the gamma 1 chain; however it lacks domain VI and domains V IV and III are shorter. It is expressed in several fetal tissues but differently from gamma 1 and is specifically localized to epithelial cells in skin lung and kidney. The gamma 2 chain together with alpha 3 and beta 3 chains constitute laminin 5 (earlier known as kalinin) which is an integral part of the anchoring filaments that connect epithelial cells to the underlying basement membrane. The epithelium-specific expression of the gamma 2 chain implied its role as an epithelium attachment molecule and mutations in this gene have been associated with junctional epidermolysis bullosa a skin disease characterized by blisters due to disruption of the epidermal-dermal junction. Two transcript variants resulting from alternative splicing of the 3' terminal exon and encoding different isoforms of gamma 2 chain have been described. The two variants are differentially expressed in embryonic tissues however the biological significance of the two forms is not known. Transcript variants utilizing alternative polyA_signal have also been noted in literature. [provided by RefSeq Aug 2011]

Gene Symbol:   LAMC2
Gene Name:   laminin, gamma 2
Aliases:   B2T, BM600, CSF, EBR2, EBR2A, LAMB2T, LAMNB2, MGC138491, MGC141938
RefSeq:   NC_000001.10 NG_007079.2 NT_004487.19
Ensembl:   ENSG00000058085
Entrez:   3918
UniGene:   Hs.591484
Chromosome Mapping:   1q25-q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 34.410000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download