PrimePCR™ SYBR® Green Assay: MIER1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021813
Assay Design:   Intron-spanning
Chromosome Location:   1:67438582-67442327question
Amplicon Length:   113
Splice Variants Targeted:   ENST00000357692 ENST00000401041 ENST00000371016 ENST00000371014 ENST00000401042 ENST00000355356 ENST00000371017 ENST00000371018 ENST00000355977

Gene Information

This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). In human this gene functions as a growth-factor-induced immediate early gene that encodes a transcriptional regulator. Depending on the cellular context this gene can function as both an activator and repressor of gene transcription. Alternative splicing results in multiple isoforms; some of which contain a nuclear localization signal. [provided by RefSeq Aug 2011]

Gene Symbol:   MIER1
Gene Name:   mesoderm induction early response 1 homolog (Xenopus laevis)
Aliases:   DKFZp781G0451, ER1, KIAA1610, MGC131940, MGC150640, MGC150641, MI-ER1, hMI-ER1
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000198160
Entrez:   57708
UniGene:   Hs.605432
Chromosome Mapping:   1p31.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.750000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download