PrimePCR™ SYBR® Green Assay: MAGI2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021762
Assay Design:   Intron-spanning
Chromosome Location:   7:77973192-77975288question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000419488 ENST00000354212 ENST00000522391 ENST00000536298 ENST00000535697 ENST00000536571

Gene Information

The protein encoded by this gene interacts with atrophin-1. Atrophin-1 contains a polyglutamine repeat expansion of which is responsible for dentatorubral and pallidoluysian atrophy. This encoded protein is characterized by two WW domains a guanylate kinase-like domain and multiple PDZ domains. It has structural similarity to the membrane-associated guanylate kinase homologue (MAGUK) family. [provided by RefSeq Jul 2008]

Gene Symbol:   MAGI2
Gene Name:   membrane associated guanylate kinase, WW and PDZ domain containing 2
Aliases:   ACVRIP1, AIP1, ARIP1, MAGI-2, SSCAM
RefSeq:   NC_000007.13 NG_011487.1 NT_007933.15
Ensembl:   ENSG00000187391
Entrez:   9863
UniGene:   Hs.603842
Chromosome Mapping:   7q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.340000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download