This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033171
PrimePCR™ PreAmp for SYBR® Green Assay: PPP1CC, Human
PrimePCR™ Template for SYBR® Green Assay: PPP1CC, Human
The protein encoded by this gene belongs to the protein phosphatase family PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes including cell division. It is expressed in mammalian cells as three closely related isoforms alpha beta/delta and gamma which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.