PrimePCR™ SYBR® Green Assay: PPP1CC, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033171

List Price:    $174.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021750
Assay Design:   Intron-spanning
Chromosome Location:   12:111160417-111162493question
Amplicon Length:   83
Splice Variants Targeted:   ENST00000335007 ENST00000340766 ENST00000551582 ENST00000553024 ENST00000546933 ENST00000550991 ENST00000551676

Gene Information

The protein encoded by this gene belongs to the protein phosphatase family PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes including cell division. It is expressed in mammalian cells as three closely related isoforms alpha beta/delta and gamma which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2011]

Gene Symbol:   PPP1CC
Gene Name:   protein phosphatase 1, catalytic subunit, gamma isozyme
Aliases:   PP-1G, PPP1G
RefSeq:   NC_000012.11 NT_009775.17
Ensembl:   ENSG00000186298
Entrez:   5501
Chromosome Mapping:   12q24.1-q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.270000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download