PrimePCR™ SYBR® Green Assay: LRTOMT, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033162

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LRTOMT, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LRTOMT, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021726
Assay Design:   Intron-spanning
Chromosome Location:   11:71804634-71806027question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000289488 ENST00000539271 ENST00000423494 ENST00000538478 ENST00000324866 ENST00000439209 ENST00000541614 ENST00000536917 ENST00000447974 ENST00000419228 ENST00000435085 ENST00000307198

Gene Information

This gene encodes two different proteins. One is a leucine-rich transmembrane protein of unknown function while the other is an O-methyltransferase. Defects in the O-methyltransferase protein can cause nonsyndromic deafness. Several transcript variants encoding different isoforms of each protein have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   LRTOMT
Gene Name:   leucine rich transmembrane and 0-methyltransferase domain containing
Aliases:   COMT2, DFNB63, FLJ52451, LRRC51, LRTOMT1, LRTOMT2
RefSeq:   NC_000011.9 NT_167190.1 NG_021423.1
Ensembl:   ENSG00000184154
Entrez:   220074
Chromosome Mapping:   11q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998000
y-intercept 35.510000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download