This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: FHL3, Human
PrimePCR™ Template for SYBR® Green Assay: FHL3, Human
The protein encoded by this gene is a member of a family of proteins containing a four-and-a-half LIM domain which is a highly conserved double zinc finger motif. The encoded protein has been shown to interact with the cancer developmental regulators SMAD2 SMAD3 and SMAD4 the skeletal muscle myogenesis protein MyoD and the high-affinity IgE beta chain regulator MZF-1. This protein may be involved in tumor suppression repression of MyoD expression and repression of IgE receptor expression. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.