This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033154
PrimePCR™ PreAmp for SYBR® Green Assay: PLCB1, Human
PrimePCR™ Template for SYBR® Green Assay: PLCB1, Human
The protein encoded by this gene catalyzes the formation of inositol 145-trisphosphate and diacylglycerol from phosphatidylinositol 45-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.