This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033120
PrimePCR™ PreAmp for SYBR® Green Assay: ADH6, Human
PrimePCR™ Template for SYBR® Green Assay: ADH6, Human
This gene encodes class V alcohol dehydrogenase which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver and it contains a glucocorticoid response element upstream of its 5' UTR which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.