PrimePCR™ SYBR® Green Assay: ADH6, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033120

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: ADH6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: ADH6, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021636
Assay Design:   Intron-spanning
Chromosome Location:   4:100129967-100131287question
Amplicon Length:   138
Splice Variants Targeted:   ENST00000394897 ENST00000394899 ENST00000237653 ENST00000508558

Gene Information

This gene encodes class V alcohol dehydrogenase which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates including ethanol retinol other aliphatic alcohols hydroxysteroids and lipid peroxidation products. This gene is expressed in the stomach as well as in the liver and it contains a glucocorticoid response element upstream of its 5' UTR which is a steroid hormone receptor binding site. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   ADH6
Gene Name:   alcohol dehydrogenase 6 (class V)
Aliases:   ADH-5
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000172955
Entrez:   130
Chromosome Mapping:   4q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.830000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download