PrimePCR™ SYBR® Green Assay: LEO1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033092

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: LEO1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: LEO1, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021566
Assay Design:   Intron-spanning
Chromosome Location:   15:52242130-52244151question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000299601 ENST00000538386 ENST00000315141

Gene Information

LEO1 parafibromin (CDC73; MIM 607393) CTR9 (MIM 609366) and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al. 2005 [PubMed 15632063]).[supplied by OMIM Mar 2008]

Gene Symbol:   LEO1
Gene Name:   Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
Aliases:   RDL
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000166477
Entrez:   123169
Chromosome Mapping:   15q21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997900
y-intercept 34.610000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download