This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Recommended - best coverage
PrimePCR™ PreAmp for SYBR® Green Assay: DBF4B, Human
PrimePCR™ Template for SYBR® Green Assay: DBF4B, Human
This gene encodes a regulator of the cell division cycle 7 homolog (S. cerevisiae) protein a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and in complex with the cell division cycle 7 homolog (S. cerevisiae) protein may facilitate M phase progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq Sep 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.