PrimePCR™ SYBR® Green Assay: EPB41, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021476
Assay Design:   Intron-spanning
Chromosome Location:   1:29365831-29379678question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000347529 ENST00000349460 ENST00000373800 ENST00000373798 ENST00000373797 ENST00000358989 ENST00000343067 ENST00000356093 ENST00000398863 ENST00000398861 ENST00000398865

Gene Information

The protein encoded by this gene together with spectrin and actin constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq Oct 2009]

Gene Symbol:   EPB41
Gene Name:   erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked)
Aliases:   4.1R, EL1, HE
RefSeq:   NC_000001.10 NT_004610.19 NG_013344.1
Ensembl:   ENSG00000159023
Entrez:   2035
UniGene:   Hs.175437
Chromosome Mapping:   1p33-p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.880000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download