PrimePCR™ SYBR® Green Assay: ATP5J, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021449
Assay Design:   Intron-spanning
Chromosome Location:   21:27097628-27102059question
Amplicon Length:   122
Splice Variants Targeted:   ENST00000400099 ENST00000400094 ENST00000284971 ENST00000457143 ENST00000400090 ENST00000400087 ENST00000400093

Gene Information

Mitochondrial ATP synthase catalyzes ATP synthesis utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core F1 and the membrane-spanning component Fo which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha beta gamma delta and epsilon) assembled in a ratio of 3 alpha 3 beta and a single representative of the other 3. The Fo seems to have nine subunits (a b c d e f g F6 and 8). This gene encodes the F6 subunit of the Fo complex required for F1 and Fo interactions. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. A pseudogene exists on chromosome Yp11.[provided by RefSeq Jun 2010]

Gene Symbol:   ATP5J
Gene Name:   ATP synthase, H+ transporting, mitochondrial Fo complex, subunit F6
Aliases:   ATP5, ATP5A, ATPM, CF6, F6
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000154723
Entrez:   522
UniGene:   Hs.246310
Chromosome Mapping:   21q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 34.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download