PrimePCR™ SYBR® Green Assay: KIFC3, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021348
Assay Design:   Intron-spanning
Chromosome Location:   16:57799496-57800824question
Amplicon Length:   66
Splice Variants Targeted:   ENST00000379655 ENST00000541240 ENST00000445690 ENST00000539578 ENST00000540079 ENST00000421376 ENST00000543930

Gene Information

KIFC3 belongs to the large superfamily of kinesins molecular motors that use the energy of ATP hydrolysis to translocate cargoes along microtubules. Members share extensive homology within a globular domain containing the microtubule- and ATP-binding sites and have a coiled-coil stalk domain that mediates oligomerization. Different kinesin family members participate in specific and diverse motile processes such as cell division organelle transport and nuclear movement (Hoang et al. 1998 [PubMed 9782090]).[supplied by OMIM Mar 2009]

Gene Symbol:   KIFC3
Gene Name:   kinesin family member C3
Aliases:   DKFZp686D23201, FLJ34694
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000140859
Entrez:   3801
UniGene:   Hs.23131
Chromosome Mapping:   16q13-q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download