This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0033015
PrimePCR™ PreAmp for SYBR® Green Assay: IQGAP1, Human
PrimePCR™ Template for SYBR® Green Assay: IQGAP1, Human
This gene encodes a member of the IQGAP family. The protein contains four IQ domains one calponin homology domain one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton with cell adhesion molecules and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.