PrimePCR™ SYBR® Green Assay: KIAA1109, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0033007

List Price:    $174.00
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PrimePCR™ PreAmp for SYBR® Green Assay: KIAA1109, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: KIAA1109, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021329
Assay Design:   Intron-spanning
Chromosome Location:   4:123264634-123265573question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000264501 ENST00000438707 ENST00000306802 ENST00000442707 ENST00000388738 ENST00000431755

Gene Information

This gene is located on the long arm of chromosome 4 in a region that is associated with susceptibility to celiac disease. The encoded protein is similar to a Chinese hamster protein that is associated with spermatocyte and adipocyte differentiation. The C-terminus of the protein is also similar to a Caenorhabditis elegans protein that plays a role in lipid storage. In mammals this protein is thought to function in the regulation of epithelial growth and differentiation and in tumor development. [provided by RefSeq Oct 2009]

Gene Symbol:   KIAA1109
Gene Name:   KIAA1109
Aliases:   DKFZp781P0474, FSA, MGC110967
RefSeq:   NC_000004.11 NG_015813.1 NT_016354.19
Ensembl:   ENSG00000138688
Entrez:   84162
Chromosome Mapping:   4q27

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 33.900000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download