This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SLC35B3, Human
PrimePCR™ Template for SYBR® Green Assay: SLC35B3, Human
Sulfation of a variety of molecules including glycoproteins proteoglycans and glycolipids requires 3-prime phosphoadenosine 5-prime phosphosulfate (PAPS) a high-energy form of the universal sulfate donor. SLC35B3 translocates PAPS from the cytosol or nucleus where it is synthesized to the Golgi lumen for use (Kamiyama et al. 2006 [PubMed 16492677]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.