This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
Info: Same primer pair as used in probe assay qHsaCIP0032962
PrimePCR™ PreAmp for SYBR® Green Assay: NQO2, Human
PrimePCR™ Template for SYBR® Green Assay: NQO2, Human
NQO2 (EC 188.8.131.52) is a flavoprotein that catalyzes the 2-electron reduction of various quinones redox dyes and the vitamin K menadione. NQO2 predominantly uses dihydronicotinamide riboside (NRH) as the electron donor (summary by Wu et al. 1997 [PubMed 9367528]).[supplied by OMIM Jul 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.