This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: BTN2A2, Human
PrimePCR™ Template for SYBR® Green Assay: BTN2A2, Human
Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6 consisting of seven genes belonging to the expanding B7/butyrophilin-like group a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.