PrimePCR™ SYBR® Green Assay: BTN2A2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021206
Assay Design:   Intron-spanning
Chromosome Location:   6:26388483-26390319question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000469230 ENST00000490025 ENST00000356709 ENST00000352867 ENST00000482842 ENST00000416795 ENST00000483410

Gene Information

Butyrophilin is the major protein associated with fat droplets in the milk. This gene is a member of the BTN2 subfamily of genes which encode proteins belonging to the butyrophilin protein family. The gene is located in a cluster on chromosome 6 consisting of seven genes belonging to the expanding B7/butyrophilin-like group a subset of the immunoglobulin gene superfamily. The encoded protein is a type I receptor glycoprotein involved in lipid fatty-acid and sterol metabolism. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2010]

Gene Symbol:   BTN2A2
Gene Name:   butyrophilin, subfamily 2, member A2
Aliases:   BT2.2, BTF2, FLJ41908
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000124508
Entrez:   10385
UniGene:   Hs.373938
Chromosome Mapping:   6p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.430000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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