PrimePCR™ SYBR® Green Assay: SLC30A4, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0021037
Assay Design:   Intron-spanning
Chromosome Location:   15:45777393-45778864question
Amplicon Length:   149
Splice Variants Targeted:   ENST00000261867

Gene Information

Zinc is the second most abundant trace metal in the human body. It is an essential element serving both a structural role as in the formation of zinc fingers in DNA-binding proteins and a catalytic role in metalloenzymes such as pancreatic carboxypeptidases (e.g. MIM 114852) alkaline phosphatases (e.g. MIM 171760) various dehydrogenases and superoxide dismutases (e.g. MIM 147450). SLC30A4 or ZNT4 belongs to the ZNT family of zinc transporters. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop (Huang and Gitschier 1997 [PubMed 9354792]).[supplied by OMIM Mar 2008]

Gene Symbol:   SLC30A4
Gene Name:   solute carrier family 30 (zinc transporter), member 4
Aliases:   ZNT4
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000104154
Entrez:   7782
UniGene:   Hs.162989
Chromosome Mapping:   15q21.1,15q21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.710000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download