This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: SLC30A4, Human
PrimePCR™ Template for SYBR® Green Assay: SLC30A4, Human
Zinc is the second most abundant trace metal in the human body. It is an essential element serving both a structural role as in the formation of zinc fingers in DNA-binding proteins and a catalytic role in metalloenzymes such as pancreatic carboxypeptidases (e.g. MIM 114852) alkaline phosphatases (e.g. MIM 171760) various dehydrogenases and superoxide dismutases (e.g. MIM 147450). SLC30A4 or ZNT4 belongs to the ZNT family of zinc transporters. ZNTs are involved in transporting zinc out of the cytoplasm and have similar structures consisting of 6 transmembrane domains and a histidine-rich cytoplasmic loop (Huang and Gitschier 1997 [PubMed 9354792]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.