PrimePCR™ SYBR® Green Assay: BCKDHB, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best coverage

List Price:    $134.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020954
Assay Design:   Intron-spanning
Chromosome Location:   6:80837291-80877403question
Amplicon Length:   98
Splice Variants Targeted:   ENST00000369760 ENST00000320393 ENST00000356489 ENST00000545529 ENST00000541767

Gene Information

Branched-chain keto acid dehydrogenase is a multienzyme complex associated with the inner membrane of mitochondria and functions in the catabolism of branched-chain amino acids. The complex consists of multiple copies of 3 components: branched-chain alpha-keto acid decarboxylase (E1) lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). This gene encodes the E1 beta subunit and mutations therein have been associated with maple syrup urine disease (MSUD) type 1B a disease characterized by a maple syrup odor to the urine in addition to mental and physical retardation and feeding problems. Alternative splicing at this locus results in transcript variants with different 3' non-coding regions but encoding the same isoform. [provided by RefSeq Jul 2008]

Gene Symbol:   BCKDHB
Gene Name:   branched chain keto acid dehydrogenase E1, beta polypeptide
Aliases:   E1B, FLJ17880, dJ279A18.1
RefSeq:   NC_000006.11 NG_009775.1 NT_007299.13
Ensembl:   ENSG00000083123
Entrez:   594
UniGene:   Hs.654441
Chromosome Mapping:   6q14.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.870000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download