This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR primer assay designed for SYBR® Green gene expression analysis.
PrimePCR™ PreAmp for SYBR® Green Assay: IL6, Human
PrimePCR™ Template for SYBR® Green Assay: IL6, Human
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. In addition the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The protein is primarily produced at sites of acute and chronic inflammation where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq Jun 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.