PrimePCR™ SYBR® Green Assay: GGN, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Recommended - best efficiency

List Price:    $134.00
Your Price:   Log In
 
Add to Custom Plate

Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0020168
Assay Design:   Intron-spanning
Chromosome Location:   19:38875011-38876128question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000334928

Gene Information

This gene is a germ cell-specific gene that encodes proteins that interact with POG (proliferation of germ cells). Alternatively spliced transcript variants of a similar mouse gene encode at least three different proteins namely gametogenetin protein 1a gametogenetin protein 2 and gametogenetin protein 3 which show a perinuclear cytoplasmic and nucleolar localization respectively. These proteins regulate the localization of POG and may play a role in spermatogenesis. [provided by RefSeq Jul 2008]

Gene Symbol:   GGN
Gene Name:   gametogenetin
Aliases:   FLJ35713, MGC33369
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000179168
Entrez:   199720
UniGene:   Hs.447948
Chromosome Mapping:   19q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.830000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
6262 PrimePCR Assays: Meeting the MIQE Guidelines by Full Wet-Lab Validation, Rev B Click to download
6263 PrimePCR Pathway Analysis: Pathway Curation and Real-Time PCR Panel Design Strategy, Rev B Click to download
6290 PrimePCR Assays and Panels Brochure, Rev D Click to download
LIT10026370 Instruction Manual, PrimePCR Assays, Panels, and Controls, Rev E Click to download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download