PrimePCR™ SYBR® Green Assay: PYROXD1, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031976

List Price:    $138.00
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Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018642
Assay Design:   Intron-spanning
Chromosome Location:   12:21598335-21602556question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000240651 ENST00000538582 ENST00000545178

Gene Information

Description Not Available

Gene Symbol:   PYROXD1
Gene Name:   pyridine nucleotide-disulphide oxidoreductase domain 1
Aliases:   FLJ22028
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000121350
Entrez:   79912
UniGene:   Hs.709545
Chromosome Mapping:   12p12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 33.550000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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