PrimePCR™ SYBR® Green Assay: CNTNAP2, Human

PrimePCR Primer Assays for Real-Time PCR oligo primer pair tube for SYBR Green gene expression

Real-time PCR primer assay designed for SYBR® Green gene expression analysis.

Info:   Same primer pair as used in probe assay qHsaCIP0031870

List Price:    $174.00
Your Price:   Log In
Quantity:  
 
Add to Custom Plate

PrimePCR™ PreAmp for SYBR® Green Assay: CNTNAP2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for SYBR® Green Assay: CNTNAP2, Human
Reaction:     200 x 20 µl reactions desalted
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding primer assay.


Assay Information

Technology:   qPCR
Assay Type:   SYBR® Green
Application:   Gene Expression
Unique Assay ID:   qHsaCID0018467
Assay Design:   Intron-spanning
Chromosome Location:   7:148106523-148112585question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000361727 ENST00000538075

Gene Information

This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein like other neurexin proteins contains epidermal growth factor repeats and laminin G domains. In addition it includes an F5/8 type C domain discoidin/neuropilin- and fibrinogen-like domains thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is directly bound and regulated by forkhead box protein P2 (FOXP2) a transcription factor related to speech and language development. This gene has been implicated in multiple neurodevelopmental disorders including Gilles de la Tourette syndrome schizophrenia epilepsy autism ADHD and mental retardation.[provided by RefSeq Mar 2010]

Gene Symbol:   CNTNAP2
Gene Name:   contactin associated protein-like 2
Aliases:   AUTS15, CASPR2, CDFE, DKFZp781D1846, NRXN4, PTHSL1
RefSeq:   NC_000007.13 NG_007092.2 NT_007914.15
Ensembl:   ENSG00000174469
Entrez:   26047
Chromosome Mapping:   7q35

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.250000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download